![]() ![]() and select Show ( ) Contents ( ) An example is shown in figure 3.6. ![]() The blastn results of the assembled contigs revealed identical 16S rRNA sequences in these two clones that best matched that of Blastococcus saxobsidens strain DD2 ( 6, 7) in GenBank, with 99.8% identity. INTRODUCTION TO CLC GENOMICS WORKBENCH 16 When you have downloaded an installer. Raw reads were trimmed and assembled into 336 and 302 contigs using the Trim Reads and De Novo Assembly tools of CLC Genomics Workbench version 20.0.4. Tutorials offer hands-on examples of how to use features of the CLC Genomics Workbench. The Online Tutorials option opens our tutorials webpage in a browser. Manuals for installed plugins can be accessed under the Plugin Help option. Genomic DNA libraries were prepared with a Nextera DNA Flex library prep kit (Illumina) and subjected to genomic sequencing using an Illumina MiSeq instrument with 2 × 150-bp pair-ended sequencing mode. The built-in Workbench user manual can be opened by choosing the Help option or by clicking on the F1 key. Genomic DNA of these two bacterial clones was prepared with DNAzol reagent (Thermo Fisher) using the manufacturer’s protocol designed for Gram-positive bacteria. These include commercial modules, such as those that are part of the QIAGEN CLC Genomics Workbench Premium product. The built-in Plugin Manager provides an up-to-date listing of the plugins available. We performed single-colony picking and isolated a clone growing mostly as motile forms, designated TML/M2B, and a clone growing mostly as aggregated nonmotile coccoid forms, designated TML/C7B (microscopic observations). The analysis functionality of the CLC Genomics Workbench can be extended substantially by installing plugins. The microorganisms grown in RPMI 1640 medium supplemented with 10% fetal bovine serum (R10) were identified as Gram-positive bacteria ( 1, 5) and formed orange-pigmented colonies on the R10 agar plates. Download CLC Genomics Workbench 20. In a prolonged laboratory tissue/cell culture experiment, we found that a flask of cell culture contained unusual microbes. ![]()
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